Journal: Frontiers in Nutrition

Article Title: Tissue specific role of ABCA1 in lung cholesterol homeostasis under high-cholesterol diet

doi: 10.3389/fnut.2025.1649407

Figure Lengend Snippet: Subcellular localization of ABCA1 in lung tissue. (A) Co-staining of ABCA1 (red) and SFTPC (green, alveolar type II marker); nuclei stained with DAPI (blue). (B) Co-staining of ABCA1 (red) and CD68 (green, macrophage marker) to determine cell-type-specific expression. n = 3–5 mice/group.

Article Snippet: Finally, DAPI was applied in the dark for 5 min, and the sections were sealed with an anti-fade mounting medium CD68 (1:100, boster), SFTPC (1:100, Proteintech), ABCA1(1:100, Proteintech).

Techniques: Staining, Marker, Expressing

Journal: Veterinary pathology

Article Title: Hemophagocytic histiocytic sarcoma in a Japanese black cow.

doi: 10.1177/0300985809358428

Figure Lengend Snippet: Figure 1. Spleen; cow. Diffuse marked splenomegaly without discrete masses. Bar ¼ 10 cm. Figure 2. Thoracic vertebrae, sagittal section; cow. A nodular mass (arrowhead) protrudes into the vertebral canal from the dorsal aspect of the fourth thoracic vertebral body. Note the dark-red zone of lysis in underlining bony tissue. Bar ¼ 2 cm. Figure 3. Splenic hemophagocytic histiocytic sarcoma; cow. Splenic red pulp is congested with numerous neoplastic histiocytes. HE. Bar ¼ 50 mm. Many neoplastic histiocytes have an eccentric nucleus and multiple phagocytized erythrocytes (inset). HE. Bar ¼ 10 mm. Figure 4. Hemophagocytic histiocytic sarcoma, fourth thoracic vertebra; cow. The neoplasm is histologically similar to that in the spleen. Fibrin exudation is evident in necrotic foci. HE. Bar ¼ 100 mm. Most of the neoplastic histiocytes have engulfed one to several erythrocytes (inset). HE. Bar ¼ 10 mm. Figure 5. Hemophagocytic histiocytic sarcoma, fourth thoracic vertebra; cow. A proportion of neoplastic histiocytes, including an erythrophagocytic cell (arrowhead), express CD68. Streptavidin– biotin–peroxidase method, with DAB chromogen and hematoxylin counterstain. Bar ¼ 50 mm.

Article Snippet: Paraffin-embedded sections of the vertebral mass and normal bovine spleen were evaluated immunohistochemically, using monoclonal antibodies against rat CD68 (clone ED1; Serotec, Oxford, UK), MHC-II (HLA-DR clone TAL.1B5; DakoCytomation, Glostrup, Denmark), CD18 (clone BAT75A; VMRD, Pullman, WA), human myeloid/histiocyte antigen (clone MAC387; DakoCytomation), human CD79a (clone HM57; DakoCytomation), polyclonal antibodies against human lysozyme (LabVision, Fremont, CA), human a-1-antitrypsin (Zymed, South San Francisco, CA), human CD3 (DakoCytomation), human CD20 (LabVision), and human lambda light chains (DakoCytomation).

Techniques: Lysis

Journal: Journal of Advanced Research

Article Title: Multi-omics approach reveals TGF-β signaling-driven senescence in periodontium stem cells

doi: 10.1016/j.jare.2024.12.037

Figure Lengend Snippet: TGF-β1-induced ROS accumulation leads to G2 arrest through ATM signaling. (A). RT-qPCR analysis of ATM signaling in TGF-β1 and/or its inhibitor-treated PDLSCs; (B). Western blot analysis of ATM signaling in TGF-β1 and/or its inhibitor-treated PDLSCs; (C). Western blot analysis of ATM signaling phosphorylated activation in the condition of ATM or CHK2 knockdown; (D). Western blot analysis of ATM signaling phosphorylated activation under N-acetyl-l-cysteine (NAC) treatment; (E). Western blot analysis of ATM signaling phosphorylated activation under decitabine treatment. Reagent doses and duration: TGF-β1 (10 ng/ml, 48 h), NAC (4 mM, 48 h), decitabine (10uM, 48 h). Predicted molecular weight: ATM 350 kDa, pATM 370 kDa, CDC25C&pCDC25C 60 kDa, CHK1&pCHK1 54 kDa, CHK2&pCHK2 62 kDa, β-Tubulin 55 kDa; GAPDH 37 kDa. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, ns denotes not significant by one-way analysis of variance followed by the Tukey’s test. Error bars represent means ± SEM of at least three independent experiments.

Article Snippet: Antibodies used in this study were listed as follows: antibodies from Abmart: anti-β-Tubulin ( M20005 ); antibodies from Huabio: anti-GAPDH recombinant rabbit monoclonal antibody (ET1601-4), anti-DNMT1 recombinant rabbit monoclonal antibody (ET1702-77), anti-DNMT3A recombinant rabbit monoclonal antibody (ET1609-31), anti-DNMT3B recombinant rabbit monoclonal antibody (ET1605-9), anti-p16 INK4A recombinant rabbit monoclonal antibody (ET1608-62), anti-AMPKγ1 recombinant mouse monoclonal antibody (EM2001-06), anti-AMPK alpha 1 recombinant rabbit monoclonal antibody (ET1608-40), anti-phospho-AMPK alpha 1 (S496) recombinant rabbit monoclonal antibody (ET1612-72), HRP-conjugated goat anti-rabbit IgG goat polyclonal antibody (HA1001), HRP-conjugated goat anti-mouse IgG polyclonal antibody (HA1006); antibodies from Abcam: anti-ATM antibody (ab32420), anti-phospho-ATM (S1981) antibody (ab81292), anti-CHK1 antibody (ab40866), anti-phospho-CHK1 (S296) antibody (ab79758), anti-CDC25C antibody (ab32444), anti-p21 antibody (ab109199), anti-CHK2 antibody (ab109413), anti-histone H3 (tri-methyl K9) (ab176916), anti-phospho-γ-H2AX (S139) antibody (ab81299), anti-HMGB1 antibody (ab79823); antibodies from CST: anti-Ki67 (D3B5) rabbit mAb (#9129), anti-phospho-CHK2 (Thr68) rabbit mAb (#2197), anti-phospho-CDC25C (Ser216) rabbit mAb (#4901); antibodies from Bioss: goat anti-rabbit IgG antibody (H + L), FITC-conjugated (bs-0295G-FITC); goat anti-rat IgG antibody (H + L), cyanine 3-conjugated (bs-0293G-Cy3); antibodies from Biolegend: APC anti-human CD34 antibody (#343509), FITC anti-human CD146 antibody (#361011).

Techniques: Quantitative RT-PCR, Western Blot, Activation Assay, Knockdown, Molecular Weight